Setlists get changed, although not on the DMB level (this also has something to do with the fact that at this point, he has a much more limited catalogue than Dave). You've got a pretty good chance of hearing something brand-new, or at the least something that's never been released.
As far as sitting/standing, the crowd will probably sit unless its GA and there's no seats. The mood is pretty sedate, but there's a good sort of energy to it. You'll feel it yourself soon enough.
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